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Biotinylated Monoclonal Mouse Anti-Human PHF-TAU, Clone AT100 (90219)

  发布日期:2010/11/9 11:02:07   浏览次数:1176
北京派胜医药科技有限公司
Beijing Pason Pharmaceuticals Inc.
Tel: (8610) 68341662/63
Fax: (8610) 68341665
Presentation
This mouse biotinylated monoclonal antibody to human PHF-tau is supplied in PBS, pH 7.4 sterile filtered (0.22 μm) and without addition of preservatives.
Source
Mouse myeloma SP2/0 cells were fused with spleen cells of a Balb/c mouse immunized intraperitoneally with partially purified human PHF-tau (1,2). This antibody has been purified from serum-free culture supernatant by protein A affinity chromatography.
Purity
The final product is more than 95% pure as determined by SDS-PAGE.
Applications
This antibody can be used for immunohistochemical staining, Western blot and ELISA techniques.
Specificity
This antibody specifically recognizes PHF-tau. No cross-reactivity with normal biopsy- or autopsy- derived tau and fetal tau has been observed (3), suggesting that AT100 detects an abnormal phophorylation site that is unique to PHF-tau. Furthermore, no signal was obtained using alkaline phosphatase-treated PHF-tau as antigen, indicating that this monoclonal is directed against a phosphatase-sensitive epitope (2). The epitope has been shown to contain the phosphorylated Ser212 and phosphorylated Thr214*.
Storage and stability
Biotinylated monoclonal mouse anti-human PHF-tau, as shipped, is stable for at least six

months when store at 4℃.

This antibody should be diluted with PBS or medium containing a suitable carrier protein (e.g. 0.1 to 1% BSA). Failure to add carrier protein to diluted product will result in loss of activity.
* numbering according to human tau40 (4).
References
(1)    Greenberg SG, Davies P. Proc Natl Acad Sci USA 1990; 87: 5827-31.
(2)    Mercken M, et al. Acta Neuropathol 1991; 84: 265-72.
(3)    Matsuo ES, et al. Neuron 1994; 13: 989-1002.
(4)    Goedert M, et al. Neuron 1989; 3: 519-26.

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