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Biotinylated Monoclonal Mouse Anti-Human PHF-TAU, Clone AT270 (90220)

  发布日期:2010/11/9 10:55:49   浏览次数:629
北京派胜医药科技有限公司
Beijing Pason Pharmaceuticals Inc.
Tel: (8610) 68341662/63
Fax: (8610) 68341665
Presentation
This mouse biotinylated monoclonal antibody to human PHF-tau is supplied in PBS, sterile filtered (0.22 μm) and without addition of preservatives.
Source
Mouse myeloma SP2/0 cells were fused with spleen cells of a Balb/c mouse immunized intraperitoneally with partially purified human PHF-tau (1). This antibody has been purified from serum-free culture supernatant by protein A affinity chromatography.
Purity
The final product is more than 95% pure as determined by SDS-PAGE.
Applications
This antibody can be used for immunohistochemical staining and ELISA techniques.
Specificity
This antibody recognizes PHF-tau, tangles and neurofilaments. Weak cross-reactivity with normal tau has been observed. Furthermore, no signal was obtained using recombinant unphosphorylated human tau as antigen.
The epitope has been determined as being the phosphorylated Thr181 residue of human tau40 (2).
Storage and stability
Biotinylated monoclonal mouse anti-human PHF-tau, as shipped, is stable for at least six months when stored at 4℃.
This antibody should be diluted with PBS or medium containing a suitable carrier protein (e.g. 0.1 to 1% BSA). Failure to add carrier protein to diluted product

will result in loss of activity.
References
(1)    Greenberg SG, Davies P. Proc Natl Acad Sci USA 1990; 87: 5827-31.
(2)    Goedert M, et al. Biochem J 1994; 301: 871-7.

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