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Monoclonal Mouse Anti-Human PHF-TAU, Clone AT8 (90206)

  发布日期:2010/11/9 10:16:16   浏览次数:1146
北京派胜医药科技有限公司
Beijing Pason Pharmaceuticals Inc.
Tel: (8610) 68341662/63
Fax: (8610) 68341665
Presentation
This mouse monoclonal antibody to human PHF-tau is supplied in PBS, sterile filtered (0.22 μm) and without addition of preservatives.
Source
Mouse myeloma SP2/0 cells were fused with spleen cells of a Balb/c mouse immunized intraperitoneally with partially purified human PHF-tau (1,2). This antibody has been purified from serum-free culture supernatant by protein A affinity chromatography.
Purity
The final product is more than 95% pure as determined by SDS-PAGE.
Applications
This antibody can be used for immunohistochemical staining (3), Western blot and ELISA techniques.
Specificity
This antibody recognizes PHF-tau and does not cross-react with normal tau as determined by a sandwich ELISA. Furthermore, no signal was obtained using alkaline phosphatase-treated PHF-tau as antigen, indicating that this monoclonal is directed against a phosphatase-sensitive epitope (2).
The epitope has been shown to contain the phosphorylated Ser202* residue (4,5).
Instructions for use
1.      For immunohistochemistry: use this antibody in a concentration range of 5-10 μg/ml for the localization of PHF-tau in formalin-fixed, paraffinembedded brain tissue.
2.      For Western blot: a final concentration of 20-60
μg/ml can detect 50 ng of SDS-denatured and

β-mercaptoethanol-reduced PHF-tau.

3. For ELISA: this antibody can be used at a concentration of 5-10 μg/ml as a capturing reagent for PHF-tau in a sandwich ELISA.
Note: The recommended concentrations are approximate values. For each application, a dose-response assay should be performed to determine the optimal concentration for use.
Storage and stability
Monoclonal mouse anti-human PHF-tau, as shipped, is stable for at least six months when stored at -20℃. Avoid multiple freeze/thaw cycles by storage in appropriate aliquots.
This antibody should be diluted with PBS or medium containing a suitable carrier protein (e.g. 0.1 to 1% BSA). Failure to add carrier protein to diluted product will result in loss of activity.
* numbering according to human tau40 (6).
References
(1)    Greenberg SG, Davies P. Proc Natl Acad Sci USA 1990; 87: 5827-31.
(2)    Mercken M, et al. Acta Neuropathol 1991; 84: 265-72.
(3)    Braak E, et al. Acta Neuropathol 1994; 87: 554-67.
(4)    Goedert M, et al. Proc Natl Acad Sci USA 1993; 90: 5066-70.
(5)    Biernat J, et al. EMBO J 1992; 11: 1593-7.
(6)    Goedert M, et al. Neuron 1989; 3: 519-26.

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